| Cat# | DIA-775 |
| Descriptions | N-Acetylglucosaminyltransferase V (MGAT5), referred to as GnT-V, catalyzes β-1,6 GlcNAc branching on the α-1,6 mannose arm of N-glycans, enhances N-glycan complexity, and is closely associated with tumor invasion, metastasis and cancer progression. |
| Applications | N-Acetylglucosaminyltransferase V (MGAT5) is widely used in β1,6 branched N-glycan detection kits and tumor invasion assay reagent development. |
| CAS No. | 24-1-15512 |
| Enzyme Commission Number | EC 2.4.1.155 |
| Enzyme Source | Trichoplusia ni, High Five (baculovirus)-derived human N-Acetylglucosaminyltrans. |
| Reaction | N4-{beta-D-GlcNAc- (1->2)-[beta-D-GlcNAc- (1->4)]-alpha-D-Man- (1->3)-[beta-D-GlcNAc- (1->2)-alpha-D-Man- (1->6)]-beta-D-Man- (1->4)-beta-D-GlcNAc- (1->4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = H+ + N4-{beta-D-GlcNAc- (1->2)-[beta-D-GlcNAc- (1->4)]-alpha-D-Man- (1->3)-[beta-D-GlcNAc- (1->2)-[beta-D-GlcNAc- (1->6)]-alpha-D-Man- (1->6)]-beta-D-Man- (1->4)-beta-D-GlcNAc- (1->4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + UDP |
| Activity Definition | Measured by its ability to transfer N-Acetyl-alpha -D-glucosamine from UDP-N-Acetyl-alpha -D-glucosamine to a biantennary N-linked core pentasaccharide in a CD39L3 coupled assay. |
| Download Datasheet: |  |
