| Cat# | EXWM-5834 |
| Source | Bovine pancreas |
| Description | DNase I is an endonuclease that digests single- or double-stranded DNA by hydrolyzing phosphodiester bonds, generating oligonucleotides with 5’-phosphate and 3’-OH ends. Its activity requires Ca2+ and is enhanced by Mg2+ or Mn2+. In the presence of Mg2+, it cuts randomly across DNA; with Mn2+, it produces blunt or sticky ends. DNase I is widely used in DNA removal from RNA preparations and in cloning workflows. This product, purified from bovine pancreas, is supplied as a freeze-dried powder with ≥ 2000 Kunitz U/mg activity. |
| Activity | ≥ 2000 Kunitz Units/mg protein |
| Synonyms | DNase I |
| Enzyme Commission Number | EC 3.1.21.1 |
| Purity | ≥ 80% by Biuret |
| Optimum pH | 7.0–8.0 |
| Inhibitors | β-Mercaptoethanol, chelating agent, SDS, actin |
| Appearance | White to light brown lyophilizate |
| Molecular Weight | ca. 31 kDa |
| Package | 15 KU/10 x 15 KU |
| Download Datasheet: |  |
