| Cat# | BDE-022 |
| Specification | 2 KU, 10 KU, 500 KU, 1000 KU |
| Description | Cholesterol Esterase (CE) hydrolyzes cholesterol esters to release free cholesterol; when used in conjunction with cholesterol oxidase, it enables the determination of total cholesterol. |
| Applications | Used in combination with cholesterol oxidase in kits for total cholesterol and lipoprotein subclass analysis. |
| Enzyme Commission Number | EC 3.1.1.13 |
| Form | White to light brown amorphous lyophilized powder |
| Source | Microorganism |
| Activity | ≥ 100 U/mg |
| Unit Definition | One unit is defined as the amount of enzyme required to catalyze the formation of 1 μmol of H₂O₂ per minute under specified assay conditions. |
| Molecular Weight | 61 kDa (SDS-PAGE) |
| Purity | ≥ 90% |
| Optimum pH | 5.0 |
| Optimum temperature | 55°C |
| pH Stability | 4.5-9.0 (37°C, 60 min) |
| Thermal Stability | < 50°C (pH 7.5, 30 min) |
| Isoelectric Point | 5.15 |
| Km Value | 5.8×10^-5 M (Cholesterol linoleate) |
| Inhibitors | Hg2+, Ag+ |
| Protectant | Sodium cholate and BSA |
| Storage | Store at -25°C to -15°C for 12 months to maintain at least 90% activity. |
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