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Native α-Glucosidase (α-Glu) from Microorganism

Cat# BDE-032
Specification 3 KU, 50 KU, 500 KU, 1000 KU
Description α-Glucosidase (α-Glu) hydrolyzes α-glycosidic bonds to release glucose, serving as a key tool for blood glucose-related metabolic analysis and enzymatic assays.
Applications Used in glycated hemoglobin and blood glucose-related assay kits to facilitate the hydrolysis and release of glycated peptide fragments.
Enzyme Commission Number EC 3.2.1.20
Form White amorphous lyophilized powder
Source Microorganism
Activity ≥ 40 U/mg
Unit Definition One unit is defined as the amount of enzyme required to generate 1 μmol of PNP per minute under specified assay conditions.
Molecular Weight 65 kDa (SDS-PAGE)
Purity ≥ 90%
Optimum pH 7.1
Optimum temperature 50°C
pH Stability 6.0-8.0 (25°C, 16h)
Thermal Stability < 50°C (pH 7.0, 10 min)
Isoelectric Point 5.2
Km Value 5.8×10^-4 M (p-Nitrophenyl-α-D-glucopyranoside)
Inhibitors SDS, Ag+, Hg2+, Cu2+
Protectant BSA
Storage Store at -25°C to -15°C for 12 months to maintain at least 90% activity.
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