| Cat# | BDE-039 |
| Description | PNGase F is an amidase that efficiently removes nearly all N-linked glycans from glycoproteins, including high-mannose, hybrid, and complex types. It cleaves the glycosidic bond between the innermost N-acetylglucosamine and asparagine. |
| Applications | Used for deglycosylation in antibody and glycoprotein drug analysis, widely applied in quality control and structural characterization. |
| Enzyme Commission Number | EC 3.5.1.52 |
| Form | Colorless liquid |
| Source | Microorganism |
| Activity | ≥ 500000 U/mL |
| Unit Definition | One unit is defined as the amount of enzyme required to remove more than 95% of the carbohydrates from 10 μg of denatured RNase B in a 10 μL reaction system over a 1-hour reaction at 37 °C under specified assay conditions. |
| Molecular Weight | 35 kDa (SDS-PAGE) |
| Purity | ≥ 95.0% by SDS-PAGE |
| Optimum pH | 7.0-8.0 |
| Optimum temperature | 65°C |
| Isoelectric Point | 8.14 |
| Inhibitors | SDS |
| Storage | Store at -25°C to -15°C. |
| Download Datasheet: |  |
