| Cat# | BDE-004 |
| Description | In the detection of non-esterified fatty acid (NEFA), the second enzymatic step employs acyl-CoA oxidase (ACO), which oxidizes acyl-CoA to 2,3-Trans-acyl-CoA and hydrogen peroxide. The latter is quantified by peroxidase and is used to measure non-esterified fatty acids. |
| Applications | Oxidizes acyl-CoA to produce hydrogen peroxide, used for the quantitative analysis of indicators related to fatty acid metabolism. |
| CAS No. | 61116-22-1 |
| Enzyme Commission Number | EC 1.3.3.6 |
| Form | White powder, lyophilized |
| Source | Recombinant |
| Activity | ≥ 30 U/mg-protein |
| Unit Definition | One unit will convert one micromole ofAcyl-CoAto trans-2, 3-dehydroacyl-CoA per min at pH 7.5 at 37°C. |
| Molecular Weight | 77 kDa (SDS-PAGE) |
| Optimum pH | 8.5 |
| Optimum temperature | 37-40°C |
| pH Stability | 6.0-8.5 (25°C, 15 h) |
| Thermal Stability | < 45°C (pH 7.5, 15 min) |
| Inhibitors | Ag+, Hg2+, Zn2+, Cu2+, Ni2+ |
| Storage | Store at ≤ -20 °C. |
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