| Cat# | POL-003 |
| MW | 90 kDa (Reducing) |
| Label | His Tag |
| Notes | It is critical to withhold Pfu DNA Polymerase until after the addition of dNTPs; otherwise, the proofreading activity of the polymerase may degrade the primers, resulting in nonspecific amplification and reduced product yield. Assemble on ice. |
| Purity | ＞95% by SDS-PAGE and HPLC |
| Source | Pyrococcus furiosus |
| Storage | Store at -25 ~ -15°C for 2 years. |
| Activity | 5 U/μL |
| Synonyms | DNA polymerase, DNA polymerase B, Pfu polymerase, Pol I |
| Component | 5 U/μL Pfu DNA Polymerase, 20 mM Tris-HCl, 0.1 mM EDTA, 0.1% Tween20, 0.1% triton X100, 1 mM DTT, 100 mM KCl, 50% Glycerol, pH 8.2 at 25°C |
| Description | Pfu DNA Polymerase is a thermostable enzyme that replicates DNA at 75°C. It catalyzes the polymerization of nucleotides into duplex DNA in the 5′→3′ direction in the presence of magnesium. The enzyme has a molecular weight of approximately 90,000 daltons as estimated from the predicted amino acid sequence and exhibits 3′→5′ exonuclease (proofreading) activity. |
| Applications | Pfu DNA Polymerase is recommended for use in PCR and primer extension reactions that require high fidelity. |
| Specification | 250 U; 1000 U |
| Dilution Buffer | 20 mM Tris-HCl, 0.1 mM EDTA, 0.1% Tween20, 0.1% triton X100, 1 mM DTT, 100 mM KCl, 50% Glycerol, pH 8.2 at 25°C |
| Expression System | E.coli |
| 10× Reaction Buffer | 200 mM Tris-HCl (pH 8.8), 20 mM MgSO₄, 100 mM KCl, 100 mM (NH₄)₂SO₄, 1% Triton X-100, 1 mg/mL nuclease-free BSA. |
| Activity Definitions | One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 74°C |
| Download Datasheet: |  |
