| Cat# | TRA-001 |
| Description | Hexokinase, derived from yeast or microorganisms, catalyzes glucose phosphorylation and serves as the core enzyme in blood glucose diagnostic kits (such as the GOD-POD method). |
| Applications | For serum glucose and creatine kinase (CK) testing |
| CAS No. | 9001-51-8 |
| Enzyme Commission Number | EC 2.7.1.1 |
| Synonyms | HK |
| Form | White amorphous powder |
| Source | Microorganism |
| Activity | ≥ 500 U/mg |
| Unit Definition | One unit will convert 1 μmol of D-Glucose to D-Glucose-6- phosphate per minute at pH 8.0 at 30°C. |
| Molecular Weight | 55 kDa (SDS-PAGE) |
| Optimum pH | 9.0 |
| Optimum temperature | 50-55°C |
| pH Stability | 5.0-7.5 (25°C, 17 h) |
| Thermal Stability | < 40°C (pH 8.0, 30 min) |
| Isoelectric Point | 5.5 |
| Km Value | 2.9×10^-3 M (Glucose), 1.5×10^-3 M (ATP) |
| Inhibitors | Co2+, Cu2+, Fe3+, Mn2+ , Ni2+, Zn2+, NEM, Proclin, SDS |
| Storage | -20°C |
| Download Datasheet: |  |
