| Cat# | TRA-044 |
| Description | Enterokinase (EK) is a highly specific serine protease that recognizes the DDDDK sequence and cleaves at the C-terminus of lysine residues (where proline cannot follow K) Hanhai Recombinant Enterokinase (GMP Grade) is expressed in E. coli and engineered from the natural enterokinase light chain fragment (containing a 6×His-tag). It exhibits enhanced specific activity and cleavage specificity, making it particularly suitable for the production of peptide-based therapeutics such as GLP-1 and its analogues. |
| Applications | Enterokinase (EK) is a highly specific serine protease that recognizes the DDDDK sequence and cleaves at the C-terminus of lysine residues (where proline cannot follow K). Hanhai recombinant enterokinase (GMP Grade) is expressed in E. coli and engineered from the natural enterokinase light chain fragment (containing a 6×His-tag). It exhibits enhanced specific activity and cleavage specificity, making it particularly suitable for the production and preparation of peptide drugs such as GLP-1 and its analogues. |
| Form | Clear, colorless to pale yellow liquid |
| Unit Definition | One unit is defined as the amount of enzyme required to cleave 95% of 0.5 mg of fusion protein stored in 25 mM Tris-HCl (pH 8.0) buffer at 25°C within 12-16 hours. |
| Purity | ≥ 95% by SDS-PAGE |
| Endotoxin Level | ≤ 10 EU/KU |
| Storage | Store at -25°C to -15°C. |
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