| Cat# | BDE-053 |
| Description | Neuraminidase (NRH) hydrolyzes N-acetylneuraminic acid residues from glycoproteins and oligosaccharides. Given the role of sialic acid (SA) as a tumor marker, enzymatic SA detection offers speed and automation compatibility. NRH initiates the enzymatic reaction in SA assay kits, making it essential for accurate measurement. |
| Applications | Used for the hydrolysis of terminal sialic acid on glycoproteins, widely applied in glycan analysis and virology research. |
| Enzyme Commission Number | EC 3.2.1.18 |
| Form | White lyophilized powder, or liquid |
| Source | Recombinant protein expression |
| Activity | 100 U/mg |
| Unit Definition | One unit is defined as the amount of enzyme required to catalyze the hydrolysis of 1 μmol of sialic acid per minute under specified assay conditions (pH 6.5, 37 °C). |
| Molecular Weight | 58 kDa (SDS-PAGE) |
| Purity | ≥ 90% by SDS-PAGE |
| Storage Buffer | 50 mM phosphate buffer, pH 6.8 |
| Storage | Store at 4°C for one year or at -20°C or -80°C for two years (sub-aliquoting is recommended to avoid repeated freeze-thaw cycles that may affect protein activity). |
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