| Cat# | DIA-846 |
| Descriptions | Recombinant Tobacco Etch Virus Protease, His-Tagged (rTEV) is fused with a polyhistidine tag for simple one-step affinity purification, specifically recognizes the ENLYFQ/S heptapeptide sequence with high cleavage specificity, and is the most commonly used tool for fusion tag removal in recombinant protein production. |
| Applications | Recombinant Tobacco Etch Virus Protease, His-Tagged (rTEV) is applied in affinity purifiable TEV protease detection kits and fusion tag removal assay reagent development. |
| CAS No. | 461-02-1816 |
| Enzyme Source | Escherichia coli |
| Synonyms | P1 Protease |
| Form | Clear colorless liquid | A 0.2 um filtered solution in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 5 mM EDTA, 10 mM GSH, with 50% Glycerol. |
| Reaction | TEV protease encoded by the tobacco etch virus is a catalytic domain of the Nuclear Inclusion a (NIa) protein. It recognizes the amino acid sequence of the general form E-X-X-Y-X-Q (or S)/X', and cleaves between Q (or S)/X'. The optimal cleavage site is ENLYFQ/G. |
| Activity Definition | One unit is defined as the amount of enzyme needed to cleave 3 mug of fusion protein in 1 hour to 85% completion at 30°C in a buffer containing 50 mM Tris-HCl, pH 8.0, 0.5 mM EDTA, and 1 mM DTT. |
| Purity | > 90% by SDS-PAGE |
| Package | 300 IU, 1000 IU |
| Download Datasheet: |  |
