| Cat# | DIA-845 |
| Descriptions | Recombinant Tobacco Etch Virus Protease S219V, GST tagged (rTEVS219V, GST) is an enhanced TEV protease mutant with improved stability and resistance to self-cleavage, carries a GST tag for convenient purification, and efficiently cleaves target proteins containing the ENLYFQG recognition sequence. |
| Applications | Recombinant Tobacco Etch Virus Protease S219V, GST tagged (rTEVS219V, GST) serves as a stable tool for fusion protein cleavage detection kits and site-specific protein processing assay reagent development. |
| CAS No. | 461-027-1816 |
| Enzyme Source | Escherichia coli |
| Form | Clear colorless liquid | A 0.2 um filtered solution in 25mM Tris-HCl, pH 8.0, 0.25 mM EDTA, 0.5Mm DTT, with 50% Glycerol. |
| Reaction | TEV protease encoded by the tobacco etch virus is a catalytic domain of the Nuclear Inclusion a (NIa) protein. It recognizes the amino acid sequence of the general form E-X-X-Y-X-Q (or S)/X', and cleaves between Q (or S)/X'. The optimal cleavage site is ENLYFQ/G. The mutant S219V was far more stable than the wild-type protease (~100-fold) and a more efficient catalyst. |
| Purity | > 90% by SDS-PAGE |
| Package | 300 IU, 1000 IU |
| Download Datasheet: |  |
