| Cat# |
POL-014 |
| Notes |
Enzymes should be stored in an ice box or on an ice bath when used, and should be stored at-20°C immediately after use. For your safety and health, please wear lab coats and disposable gloves for operation. |
| Storage |
Store at -25 ~ -15°C for 1 year. |
| Activity |
2000 U/μL |
| Features |
It has strong thermal stability, which is helpful for the amplification of complex templates and improves the specificity of the system. |
| Component |
10 mmol/L Tris-HCl, 50 mmol/L KCl, 0.1 mmol/L EDTA, 1 mmol/L DTT, 0.1% Triton X-100, 50% Glycerol, pH7.5 at 25°C |
| Description |
Super Bst DNA Polymerase is derived from Thermophilic Geobacillus sp. DNA Polymerase I, genetically engineered to remove its 5′→3′ exonuclease activity. This product exhibits strong 5′→3′ DNA polymerase activity, strand displacement activity, and dUTP tolerance, making it more suitable for contamination-resistant isothermal amplification reactions such as LAMP and CPA. Compared to Bst Plus DNA Polymerase, this product improves the thermostability of the Bst enzyme, contributing to enhanced amplification performance. This enzyme is suitable for both low-template and high-temperature LAMP reaction systems, effectively improving detection specificity and shortening detection time. Its thermostability also makes it suitable for developing hot-start Bst, further enhancing detection specificity. |
| Specification |
20000 U; 100000 U; 200000 U |
| Activity Definitions |
1 Urefers to the amount of enzyme required to incorporate 10 nmol of dNTP into the acid-insoluble precipitate in 30 min at 65°C |
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