| Cat# | TRA-051 |
| Specification | 1KU,5KU,20KU |
| Description | Creatine kinase (CK) catalyzes the reversible transfer of phosphate between ATP/ADP and the creatine/creatine phosphate system. Four major isoforms exist: two cytoplasmic forms (MM-CK and BB-CK) and two mitochondrial forms (Miu-CK and Mis-CK), forming dimers and octamers, respectively. CK maintains energy balance in high-metabolism tissues such as skeletal muscle, buffering cells against rapid ATP depletion. |
| Applications | Biochemical research; (Reversible) catalysis converting ADP and phosphocreatine into ATP and creatine; for ATP production; providing energy regeneration for mixtures of sperm, spermatogonial cell, and oocyte extracts; for analysis of Golgi body degradation and reorganization; for determination of CK substrates; also used for recombinase polymerase amplification (RPA). |
| Synonyms | Creatine kinase from rabbit muscle; Creatine kinase from rabbit muscle; Creatine kinase from rabbit muscle; Creatine kinase M-type; Creatine kinase M chain; Creatine phosphokinase; Creatine phosphokinase M-type; Phosphocreatine phosphokinase; Creatine phosphotransferase; Creatine N-phosphotransferase; ATP:creatine N-phosphotransferase; Adenosine-5-triphosphate |
| Form | Lyophilized (freeze-dried) powder |
| Species | Rabbit |
| Source | Rabbit muscle |
| Activity | ≥ 250 U/mg |
| Unit Definition | One unit will transfer 1 μmol of phosphate from phosphocreatine to ADP per minute at pH 7.4 at 30°C. |
| Molecular Weight | 81 kDa (Dimer) |
| Purity | ＞95% by SDS-PAGE; no detectable DNase and RNase; Pyruvate kinase ＜1 mU/mg (＜0.001%); LDH ＜0.1 mU/mg (＜0.001%). |
| Gene ID | 100009056 |
| Accession | P00563 |
| Storage | Store at -20°C: Valid for 2 years. Store at 4°C for 7 days shows no significant decrease in activity. Store at room temperature or 37°C for 3 days reduces activity by approximately 10%. |
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