| Cat# | ENZD-QM10 |
| Specification | 500 rxns（20 μl/rxn） 2500 rxns（20 μl/rxn） |
| Aplications | Gene Expression Analysis; High Sensitivity qPCR Reaction |
| Description | Suitable for probe-based qPCR detection of DNA templates, with high sensitivity and good amplification efficiency. It has an integrated Heat-labile dUTP/UDG anti-contamination system, effectively eliminating the impact of amplification product contamination. |
| Features | 1. Excellent amplification sensitivity: Based on hot-start AceTaq DNA Polymerase, providing excellent amplification performance and sensitivity 2. Reliable quantitative results: Introduction of Heat-labile UDG anti-contamination system, effectively preventing PCR product contamination 3. Robust repeatability: Reliable quantitative results, meeting the data requirements of high-level journals |
| Components | 2 × U Probe Master Mix\n50 × ROX Reference Dye 1\n50 × ROX Reference Dye 2 |
| Shipping and Storage Conditions | Store at -20℃ in the dark |
| Download Datasheet: |  |
Uracil-containing DNA templates at concentrations of 0 ng, 0.004 ng, 0.04 ng, 0.4 ng, and 4 ng were introduced into qPCR reaction systems based on ENZD-QM09 and ENZD-QM10 to evaluate contamination removal efficiency.
Across increasing template inputs, ENZD-QM10 demonstrated effective suppression of carryover nucleic acid signals, with CT values remaining stable and no observable interference from contaminating templates. This indicates a highly reliable U+ contamination control mechanism capable of maintaining assay accuracy under varying contamination loads.
In addition, the formulation exhibited excellent storage stability, ensuring consistent performance of the anti-contamination system during routine experimental handling. Data sourced from internal validation studies evaluating uracil-containing template removal efficiency in qPCR systems.
