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Glutamine Synthetase (GST) from Microorganism

Cat# DIA-433
Source Microorganism
Form Freeze dried powder
Activity ≥ 15.0 U/mg
Isoelectric point 6.5
Unit Definition One unit (U) is defined as the amount of enzyme which produces 1 μmol of phosphate per min at 37 °C and pH 7.0 under the conditions described below.
Storage Below –20 °C
Enzyme Commission Number EC 6.3.1.2
pH Stability 6.5–9.5
Michaelis Constant 1.5 × 10-2 M (L-glutamate)
1.3 × 10-4 M (Ammonia)
8.7 × 10-4 M (ATP)
Optimum pH ca. 7.0
Optimum temperature 60 °C
Thermal stability Below 40 °C
Inhibitors Methionine sulfoximine, carbamyl phosphate
Contaminants Catalase ≦ 0.5 U/U%
Applications For the determination of ammonia and ATP in clinical analysis.
Appearance White to light yellow lyophilizate
Molecular Weight ca. 900 kDa (Gel filtration)
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