| Cat# | DIA-478 |
| Source | E. coli |
| Description | Expressed in E. coli, ca. 50 kDa. Specifically inhibits RNase A, B, and C by forming 1:1 complexes, reversible by urea or thiol reagents. Superior antioxidant activity compared to human RNase inhibitor, suitable for experiments sensitive to DTT. Glycerol-free, lyophilization compatible. |
| Unit Definition | One unit (U) is defined as the amount of enzyme required to inhibit 50% of the activity of 5 ng RNase A. (RNase A activity is quantified by the inhibition of cyclic 2′,3′-CMP hydrolysis to 3′-CMP.) |
| pH Stability | 5.0–9.0 |
| Optimum pH | 7.0–8.0 |
| Thermal stability | Below 65°C |
| Applications | Suitable for any experiment requiring RNase-free conditions to prevent RNA degradation, such as cDNA synthesis, in vitro translation, polysome isolation, and cell-free transcription. |
| Product Overview | Product Components: 3 KU: 40 U/μL – 75 μL 15 KU: 40 U/μL – 375 μL 2 KU: 200 U/μL – 10 μL |
| Molecular Weight | 50 kD |
| Package | 3/15/2 KU |
| Download Datasheet: |  |
