| Cat# | ENZD-P01 |
| Specification | 1000 U; 4000 U |
| Unit Concentration | 20 U/μl |
| Description | Heat-labile UDG (Glycerol-free) is a glycerol-free version of Heat-labile UDG, combining the feasibility of lyophilization with the excellent removal performance and stability of glycerol-containing UDG enzymes for U-template contamination. This product contains no other components that affect lyophilization, making it suitable for the development of lyophilized products. It exhibits high activity at room temperature, and 50°C for 2 minutes irreversibly inactivates most enzymes. It is suitable for PCR, qPCR, RT-PCR, and RT-qPCR systems. |
| Features | Rapid inactivation at 50℃ Compatible with common PCR, qPCR, RT-PCR, and RT-qPCR reaction systems |
| Applications | Remove dU-containing PCR product aerosol contamination; remove uracil bases from single-stranded or double-stranded DNA |
| Components | Heat-labile UDG, Glycerol-free, 20 U/μl |
| Quality Control | Strong cleaning ability; stable freeze-drying performance; Thermosensitive UDG enzyme |
| Shipping and Storage Conditions | Store at -30 ~ -15℃, transport at ≤0℃. |
| Download Datasheet: |  |
Evaluated using three concentrations of dU-containing templates, ENZD-P01 was tested in parallel with Supplier A, using a no-UDG control as reference. As a diagnostic grade UDG, ENZD-P01 demonstrated stronger clearance capability under identical template concentrations, more effectively removing non-template DNA contamination from the system.Data sourced from internal validation studies, supporting the consistency and purity of the enzyme performance.
Performance was assessed across different concentrations of uracil-containing templates before and after lyophilization of ENZD-P01. Results showed that, compared with the no-UDG control, the enzyme effectively degraded templates both before and after freeze-drying, with consistent amplification performance. Data sourced from internal validation studies, confirming stable performance and high purity after lyophilization.
ENZD-P01, an Antarctic-derived thermolabile UDG from Creative Enzymes, was systematically evaluated for its thermal inactivation performance. Incubation at 50°C for 2 minutes resulted in irreversible inactivation of most enzyme activity, while 5 minutes of incubation led to complete inactivation.Data sourced from internal validation studies, demonstrating reliable inactivation behavior with consistent results.
