| Cat# | ENZD-RT13 |
| Specification | 250 rxns（20 μl/rxn） |
| Aplications | Virus Detection; Pathogen Detection; Gene Expression Analysis |
| Description | Uses an optimized dUTP/dNTP system and adds Heat-labile UDG, which degrades contaminating DNA at room temperature and inactivates rapidly at 50–55°C, ensuring cDNA integrity. Combines anti-contamination with high-sensitivity detection, suitable for RNA template quantitative analysis. |
| Features | 1. Supports multiplex probe detection. 2. UDG anti-contamination system: Heat-labile UDG rapidly degrades U-containing double-stranded DNA contaminants at room temperature, and Heat-labile UDG quickly inactivates at 50-55°C, maintaining the integrity of cDNA. 3. High detection sensitivity: Able to detect templates with a few copies or 0.1 pg total RNA. |
| Components | RNase-free ddH2O 5 × One Step U+ Mix One Step U+ Enzyme Mix 50 × ROX Reference Dye 1 50 × ROX Reference Dye 2 |
| Shipping and Storage Conditions | Store at -20℃ in the dark |
| Download Datasheet: |  |
The ENZD-RT13 one-step RT-qPCR probe kit from Creative Enzymes incorporates an optimized dUTP/UDG contamination control system, combining controlled dUTP incorporation with Heat-labile UDG.
This design enables efficient degradation of carryover DNA while preserving amplification performance, ensuring reliable contamination control without compromising reaction efficiency. Data sourced from internal validation studies evaluating dUTP/UDG system design and performance.
