Inorganic pyrophosphatase (PmPPA) from Pasteurella multocida catalyzes the hydrolysis of inorganic pyrophosphate into two orthophosphate molecules, drives thermodynamically unfavorable biosynthetic reactions forward, and is commonly added to coupled enzymatic reaction systems.
Applications
Inorganic pyrophosphatase (PmPPA) is widely applied in coupled enzymatic reaction detection kits and biosynthetic reaction efficiency enhancement reagent development.
CAS No.
36-1-12
Enzyme Commission Number
EC 3.6.1.1
Enzyme Source
E.coli recombinant inorganic pyrophosphatase from Pasteurella multocida.
Reaction
P2O7-4+H2O = 2HPO4-2
Activity Definition
One unit is defined as the amount of enzyme that catalyzes the formation of 1 Mumo inorganic phosphate from inorganic pyrophosphate per minute at 37°C.