| Cat# | DIA-907 |
| Descriptions | α-Mannosidase hydrolyzes α-linked mannose glycosidic bonds in high-mannose and complex type N-glycans, participates in the stepwise processing of N-linked glycan maturation, and acts as an essential tool enzyme for glycoprotein glycan trimming and mannose metabolism research. |
| Applications | α-Mannosidase is applied in N-glycan trimming detection kits and mannose metabolism pathway assay reagent development. |
| CAS No. | 9025-42-7 |
| Enzyme Commission Number | EC 3.2.1.24 |
| Molecular Weight | Approximately 220 kDa |
| Synonyms | ALPHA-MANNOSIDASE; ALPHA-D-MANNOSIDE MANNOHYDROLASE; ALPHA1,6-MANNOSIDASE; ALPHA1-2,3-MANNOSIDASE; EC3.2.1.24; a1,2Mannoisdase,Trichodermareesei,Recomb.,Saccharomyces; a1,6-Mannosidase,Xanthomonassp.,Recomb.,E.coli; a1-2,3-Mannosidase,Recomb.,E.coli |
| Form | Suspension in 50 mM PB buffer, pH 6.5. |
| Activity | ≥ 30 U/mg protein |
| Activity Definition | One unit of activity is defined as the amount of enzyme that released 1 μmol of p-nitrophenol per min at pH 6.0 and 60°C using p-nitrophenyl α-D-mannopyranoside as a substrate. |
| Assay | Michaelis constant: 5.2 ×10-4mol/L (p-nitrophenyl α-D-mannopyranoside) | Physical form: Suspension in 50 mM PB buffer | pH 6.5 | 6.0 | 70 °C | 5~7 (25 °C | 24 hrs) | 60 °C (pH 6.0 | 24hrs) | EDTA | swainsonine |
| Storage | Store at 2-8°C for 2 years. Sealed and away from light. |
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