| Cat# | BDE-021 |
| Specification | 2 KU, 10 KU, 500 KU, 1000 KU |
| Description | Cholesterol Oxidase (CO) catalyzes the oxidation of cholesterol to produce hydrogen peroxide and is a core enzyme in lipid profile testing. |
| Applications | Core enzyme for total cholesterol and high-density lipoprotein (HDL) cholesterol assay kits. |
| Enzyme Commission Number | EC 1.1.3.6 |
| Form | Yellow amorphous lyophilized powder |
| Source | Microorganism |
| Activity | ≥ 8 U/mg |
| Unit Definition | One unit is defined as the amount of enzyme required to catalyze the production of 1 μmol of H₂O₂ per minute under specified assay conditions. |
| Molecular Weight | 60 kDa (SDS-PAGE) |
| Purity | ≥ 90% by SDS-PAGE |
| Optimum pH | 7.0 |
| Optimum temperature | 50°C |
| pH Stability | 5.5-10 (25°C, 16 h) |
| Thermal Stability | < 40°C (pH 7.0, 30 min) |
| Isoelectric Point | 5.6 |
| Km Value | 3.3×10^-4 M (Cholesterol) |
| Inhibitors | SDS, Ag+, Zn2+, Hg2+ |
| Protectant | BSA and nonionic surfactants |
| Storage | Store at -25°C to -15°C for 12 months to maintain at least 90% activity. |
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