| Cat# | BDE-015 |
| Description | Creatine Amidinohydrolase acts on creatine, providing a critical upstream catalytic step for creatinine metabolism assays. |
| Applications | Used in combined creatinine and creatine assay kits to enable precise analysis of renal function indicators. |
| Enzyme Commission Number | EC 3.5.3.3 |
| Form | White amorphous lyophilized powder |
| Source | Microorganism |
| Activity | ≥ 5.8 U/mg |
| Unit Definition | One unit is defined as the amount of enzyme required to catalyze the complete conversion of 1 μmol of creatine to creatine monophosphate per minute under specified assay conditions. |
| Molecular Weight | 48 kDa (SDS-PAGE) |
| Purity | ≥ 90% |
| Optimum pH | 8.0 |
| Optimum temperature | 45°C |
| pH Stability | 4.5-10.0 (25°C, 16 h) |
| Thermal Stability | < 50°C (pH 7.5, 30 min) |
| Isoelectric Point | 4.6 |
| Km Value | 8.81×10^-3 M (Creatinine) |
| Inhibitors | Ag+, Cu2+, Hg2+ |
| Protectant | EDTA and carbohydrates |
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