| Cat# |
BDE-034 |
| Specification |
1 KU, 10 KU, 50 KU |
| Description |
Fructosyl-peptide Oxidase (FPOX) specifically acts on glycated peptide segments, enabling highly specific enzymatic detection of glycated hemoglobin. |
| Applications |
Used in enzymatic HbA1c (glycated hemoglobin) assay kits to enable highly specific direct measurement. |
| Enzyme Commission Number |
EC 1.5.3 |
| Form |
Yellow amorphous lyophilized powder |
| Source |
Microorganism |
| Activity |
≥ 4 U/mg |
| Unit Definition |
One unit is defined as the amount of enzyme required to catalyze the production of 1 μmol of H₂O₂ per minute under specified assay conditions. |
| Molecular Weight |
60 kDa (SDS-PAGE) |
| Purity |
≥ 90% by SDS-PAGE |
| Optimum pH |
6.5-7.5 |
| Optimum temperature |
37°C |
| pH Stability |
6.5-9.5 (25°C, 16 h) |
| Thermal Stability |
< 40°C (pH 8.0, 30 min) |
| Isoelectric Point |
6.4 |
| Km Value |
4.0×10^-3 M (fructosyl-Val-His) |
| Inhibitors |
Hg2+, Pb2+ |
| Protectant |
Glycerin, trehalose |
| Storage |
Store at -25°C to -15°C for 12 months to maintain at least 90% activity. |
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