α-L-Fucosidase (AFU) hydrolyzes fucosyl-containing substrates and serves as a marker enzyme for the diagnosis of important diseases such as hepatocellular carcinoma.
Applications
Used in hepatocellular carcinoma diagnosis kits as the enzyme for the direct detection of the serum biomarker α-L-fucosidase.
Enzyme Commission Number
EC 3.2.1.51
Form
Colorless transparent liquid
Source
Microorganism
Activity
≥ 5 U/mL
Unit Definition
One unit is defined as the amount of enzyme required to hydrolyze 1 μmol of CNP-AFU per minute under specified assay conditions.
Molecular Weight
55 kDa (SDS-PAGE)
Purity
≥ 90%
Optimum pH
7.5
Optimum temperature
80°C
pH Stability
7.0-10.0 (25°C, 16 h)
Thermal Stability
< 90°C (pH 8.0, 30 min)
Isoelectric Point
6.2
Storage
Store at -25°C to -15°C for 12 months to maintain at least 90% activity.