diagnostic-enzymes

Native Microorganism N-Acetylneuraminic acid aldolase

Native Microorganism N-Acetylneuraminic acid aldolase

Cat# SimilarSourceDescriptionFormActivityCAS No.Isoelectric pointSynonymsEnzyme Commission NumberpH StabilityMichaelis ConstantOptimum pHOptimum temperatureThermal stabilityStabilityInhibitorsContaminantsAbbrAliasApplicationsAppearanceStructureMolecular Weight
DIA-182 NALaseMicroorganismIn enzymology, a N-acetylneuraminate lyase (EC 4.1.3.3) is an enzyme that catalyzes the chemical reaction: N-acetylneuraminate ↔ N-acetyl-D-mannosamine + pyruvate. Hence, this enzyme has one substrate, N-acetylneuraminate, and two products, N-acetyl-D-mannosamine and pyruvate. This enzyme belongs to the family of lyases, specifically the oxo-acid-lyases, which cleave carbon-carbon bonds.Freeze dried powderGrade III 15U/mg-solid or more (30U/mg-protein or more), (containing approx. 30% of stabilizers)9027-60-54.6±0.1N-Acetylneuraminate Pyruvate Lyase; N-Acetylneuraminic Acid Lyase; NANA Aldolase; EC 4.1.3.3; N-acetylneuraminate pyruvate-lyase (N-acetyl-D-mannosamine-forming); N-acetylneuraminic acid aldolase; acetylneuraminate lyase; sialic aldolase; sialic acid aldolase; sialate lyase; N-acetylneuraminic aldolase; neuraminic aldolase; N-acetylneuraminate aldolase; neuraminic acid aldolase; N-acetylneuraminic acid aldolase; neuraminate aldolase; N-acetylneuraminic lyase; NPL; NALase; NANA lyase; acetylneuraminate pyruvate-lyase; N-acetylneuraminate pyruvate-lyaseEC 4.1.3.3pH 6.0−9.0 (10°C, 25hr)2.5×10⁻³M (N-Acetylneuraminic acid)7.5-8.070°Cbelow 65°C (pH 7.5, 30min)Stable at-20°C for at least 6 monthsp-Chloromercuribenzoate, sodium dodecyl sulfate, Hg⁺⁺, Ag⁺Catalase < 1.0%, NADH oxidase < 1.0x10⁻³%NALase (Microorganism)NPL; NALase; NANA lyaseThis enzyme is useful for enzymatic determination of N-acetylneuraminic acid and sialic acid when coupled with the related enzymes in clinical analysis. For industrial use, this enzyme is useful for enzymatic synthesis of sialic acid.Yellowish amorphous powder, lyophilized3 subunits (approx. 35,000) per mol of enzymeapprox. 98 kDa Download Datasheet:
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