| Cat# | BDE-003 |
| Description | In non-esterified fatty acid (NEFA) detection, acyl-CoA synthetase (ACS) catalyzes the first reaction, converting free fatty acids to acyl-CoA, AMP, and pyrophosphate in the presence of coenzyme A and ATP. |
| Applications | Catalyzes the activation of fatty acids to form acyl-CoA, acting as the key initiating enzyme in the free fatty acid enzymatic assay. |
| CAS No. | 9013-18-7 |
| Enzyme Commission Number | EC 6.2.1.3 |
| Form | White powder, lyophilized |
| Source | Recombinant |
| Activity | ≥ 5 U/mg-protein |
| Unit Definition | One unit will convert one micromole of fatty acid to acyl-CoA per min at pH 7.5 at 37°C. |
| Molecular Weight | 77 kDa (SDS-PAGE) |
| Optimum pH | 7.5 |
| Optimum temperature | 50°C |
| pH Stability | 6.5-7.5 (25°C, 18 h) |
| Thermal Stability | < 45°C (pH 7.5, 10 min) |
| Inhibitors | Ag+, Hg2+, Fe3+, Zn2+, Cu2+ |
| Storage | Store at ≤ -20 °C. |
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