| Cat# | BDE-050 |
| Description | Cystathionine β-synthase (CBS) is a cytoplasmic homotetramer (63 kDa subunits) and a PLP-dependent enzyme that condenses serine and homocysteine to form cystathionine. As a key regulator of Hcy levels, CBS is essential in the transsulfuration pathway and is used in enzymatic cycling assays for Hcy detection. |
| Applications | Used in homocysteine assay kits as a key coupling enzyme in a cyclic amplification detection system. |
| Enzyme Commission Number | EC 4.2.1.22 |
| Form | Slightly yellow, yellow powder, or liquid |
| Source | Recombinant microbial proteins |
| Activity | 250 U/mg |
| Unit Definition | One unit is defined as the amount of enzyme required to catalyze a reaction that causes a decrease of 0.01 in the absorbance of NADH at 340 nm per minute under specified assay conditions (37 °C, pH 8.0). |
| Molecular Weight | 56 kDa (SDS-PAGE) |
| Purity | ≥ 90% by SDS-PAGE |
| Optimum pH | 7.4-8.5 |
| Isoelectric Point | 6.49 |
| Storage Buffer | Phosphate buffer, pH 7.4 |
| Storage | Store at 4°C for one year; store at -20°C or -80°C for at least two years. Avoid repeated freeze-thaw cycles as much as possible. |
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