| Cat# | Kit-047 |
| Description | This multiplex PCR–based RNA library preparation kit is designed for sequencing analysis of viral RNA genomes. It includes a complete reverse transcription module and a multiplex PCR–based library construction module, employing a two-round PCR strategy for targeted genome amplification and enrichment. From viral RNA input to sequencing-ready libraries, the entire workflow can be completed in as little as 5 hours, with hands-on time under 1.5 hours. The kit supports input samples with Ct values ranging from 16 to 38, and the resulting libraries are suitable for sequencing on Illumina high-throughput platforms. Customized panels included with this kit achieve up to 99.7% coverage of viral genomes, enabling effective detection of genomic mutations and deletions. Optimized reagent combinations deliver stable and reliable performance, supporting rapid and high-quality viral RNA library construction. All reagents are subjected to strict quality control and functional validation to ensure excellent stability and reproducibility of library preparation. |
| Storage | -30 °C to -15 °C |
| Applications | Genetic analysis of viral RNA genomes; viral RNA library preparation. |
| Product Overview | Simple operation: Two-round PCR method, entire workflow completed in as little as 5 hours. Excellent coverage: Custom panel achieves 99.7% coverage of viral genomes. |
| Package | 24 rxns 10× RT Mix: 48 μL HiScript Ill Enzyme Mix: 48 μL Random Hexamers: 24 μL RNase-free ddH2O: 1 mL 4× Multi-PCR Mix: 240 μL Primer Pool 1: 48 μL Primer Pool 2: 48 μL HiFi Amplification Mix: 2 x 480 μL TE: 1 mL |
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