| Cat# | TRA-061 |
| Specification | 100U,500U |
| Description | T4 polynucleotide kinase (T4 PNK) catalyzes the transfer of the γ-phosphate from ATP to the 5′-hydroxyl group of DNA, RNA, oligonucleotides, or mononucleotides. It accepts other NTPs as phosphate donors. |
| Applications | 5'-end labeling of oligonucleotides, DNA, or RNA for use as probes in Southern, Northern, EMSA, etc.; as markers in gel electrophoresis; as DNA sequencing primers; as PCR primers; etc. 5'-end phosphorylation of oligonucleotides, DNA, or RNA to ensure smooth subsequent ligation reactions; Catalyze 5'-phosphorylation of mononucleotides for 3'-phosphorylation, enabling their ligation to the 3'-terminus of DNA or RNA; remove the 3'-phosphate group. |
| Source | E.coli |
| Unit Definition | One unit is defined as the amount of enzyme required to transfer 1 nmol of γ-phosphate groups from ATP to the 5'-OH terminus of DNA within 30 minutes at 37°C. |
| Storage Buffer | 20 mM Tris-HCl (pH 7.5), 25 mM KCl, 0.1 mM EDTA, 2 mM DTT, 50% Glycerol. |
| Storage | Store at -20°C. |
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