| Cat# | DIA-776 |
| Descriptions | N-Acetylglucosaminyltransferase1 (MGAT1), namely GnT-I, transfers the first N-acetylglucosamine to the α-1,3 mannose residue of high-mannose N-glycans, and represents the indispensable initiating enzyme for hybrid and complex N-glycan biosynthesis. |
| Applications | N-Acetylglucosaminyltransferase1 (MGAT1) serves as an essential tool for hybrid and complex N-glycan detection kits and glycosylation pathway assay reagent development. |
| CAS No. | 24-1-1019 |
| Enzyme Commission Number | EC 2.4.1.101 |
| Enzyme Source | Trichoplusia ni, High Five (baculovirus)-derived human N-Acetylglucosaminyltrans. |
| Reaction | N4- (alpha-D-Man- (1->3)-[alpha-D-Man- (1->3)-[alpha-D-Man- (1->6)]-alpha-D-Man- (1->6)]-beta-D-Man- (1->4)-beta-D-GlcNAc- (1->4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glycan mannose isomer 5A1, 2) + UDP-N-acetyl-alpha-D-glucosamine = H+ + N4-{beta-D-GlcNAc- (1->2)-alpha-D-Man- (1->3)-[alpha-D-Man- (1->3)-[alpha-D-Man- (1->6)]-alpha-D-Man- (1->6)]-beta-D-Man- (1->4)-beta-D-GlcNAc- (1->4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + UDP |
| Activity Definition | Measured by its ability to transfer N-Acetyl-D-Glucosamine from UDP-GlcNAc to alpha 1-3, alpha 1-6-Mannotriose. |
| Download Datasheet: |  |
