| Cat# | DIA-543 |
| Source | Escherichia coli |
| Description | High purity recombinant phosphomannose isomerase from Escherichia coli for use in research, biochemical enzyme assays and in vitro diagnostic analysis. |
| Form | Suspension |
| CAS No. | 9023-88-5 |
| Activity | ~ 89 U/mg (25 °C, pH 7.6 on mannose 6-phosphate) |
| Unit Definition | One unit of phosphomannose isomerase activity is defined as the amount of enzyme required to release one µmole of fructose 6-phosphate from mannose 6-phosphate (3.14 mM) per minute in the presence of NAD+ at pH 7.6 and 25 °C. |
| Storage | 2–8 °C |
| Synonyms | Mannose-6-phosphate isomerase; D-mannose-6-phosphate aldose-ketose-isomerase |
| Enzyme Commission Number | EC 5.3.1.8 |
| Stability | > 1 year under recommended storage conditions |
| Optimum pH | 7.6 |
| Optimum temperature | 40 °C |
| Buffer | 3.2 M ammonium sulphate |
| Applications | Applications for the measurement of D-mannose in carbohydrate research and in the food and feeds, fermentation, wine, beverage and dairy industries. |
| Molecular Weight | 43915 Da |
| Concentration | ~ 500 U/mL |
| Specificity | Catalyses the reaction: D-Mannose 6-phosphate = D-fructose 6-phosphate. |
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