| Cat# |
TRA-560 |
| Specification |
5μg(min) |
| Description |
Full-length human Erk1 was produced as an N-terminal GST fusion protein 70 kDa using an E. coli expression system. GST-Erk1 was purified by glutathione sepharose chromatography and then activated with His-tagged MAP2K1. The activated GST-Erk1 was re-purified using glutathione sepharose chromatography. |
| Applications |
For use as a positive control in ERK1 detection assays for MAPK pathway research. |
| Synonyms |
MAPK3, ERK-1, ERK1, ERT2, HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, PRKM3, p44-ERK1, p44-MAPK, p44erk1, p44mapk |
| Source |
E. coli |
| Molecular Weight |
70 kDa |
| Class |
Serine/Threonine Kinase |
| Group |
CMGC |
| Family |
MAPK |
| Gene Name |
MAPK3 | mitogen-activated protein kinase 3 |
| Sequence |
1-379 (end) |
| Accession Number |
NP_002737.2 |
| Tag |
GST-Tag |
| Tag Location |
N-terminal GST |
| Storage Buffer |
50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35,1 mM DTT, 10% glycerol, pH 7.5 |
| Storage |
Store at -80°C. Avoid repeating freeze-thaws. |
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