| Cat# | BDE-055 |
| Description | 3α-Hydroxysteroid dehydrogenase (HSD) is a steroid dehydrogenase that reversibly catalyzes the oxidation/reduction of 3-hydroxy/keto groups in C19–27 steroids. It has an optimal pH of 7–9, an isoelectric point of 4.8, and is inhibited by Hg²⁺ and Ag⁺ but remains stable with EDTA. |
| Applications | Used in total bile acid and steroid hormone detection kits to enable highly sensitive enzymatic assays. |
| Enzyme Commission Number | EC 1.1.1.50 |
| Form | White lyophilized powder |
| Source | Recombinant protein expression |
| Activity | 50 U/mg |
| Unit Definition | One unit is defined as the amount of enzyme required to catalyze the oxidation of 1 μmol of androstenone per minute under specified assay conditions (25 °C, pH 8.9). |
| Molecular Weight | 28 kDa (SDS-PAGE) |
| Purity | ≥ 90% by SDS-PAGE |
| Storage Buffer | 20 mM Tris, pH 8.0 |
| Storage | Freeze-dried powder: Store at 4°C for one year or at -20°C for two years (sub-aliquoting is recommended to avoid repeated freeze-thaw cycles). |
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