| Cat# | DIA-465 |
| Description | An engineered M-MuLV reverse transcriptase with reduced RNase H activity and enhanced thermal stability. Compared to wild-type M-MuLV, it can synthesize first-strand cDNA at higher temperatures. Active even at 48 °C, it offers higher specificity, greater yield, and can generate cDNA up to 12 kb. |
| Storage | –20 °C |
| Applications | First-strand full-length cDNA synthesis; the resulting cDNA can be used for PCR, real-time PCR, second-strand cDNA synthesis, and more. |
| Product Overview | Product Components: 4000 U: Reverse Transcriptase II (200,000 U/mL): 20 μL 5x First-Strand Buffer: 500 μL 100 mM DTT (10x): 200 μL 10000 U: Reverse Transcriptase II (200,000 U/mL): 50 μL 5x First-Strand Buffer: 500 μL 100 mM DTT (10x): 200 μL 200000 U: Reverse Transcriptase II (200,000 U/mL): 1 mL 5x First-Strand Buffer: 10 mL 100 mM DTT (10x): 1.25 mL x 2 Product Features: Low RNase H activity enables cDNA synthesis up to 12 kb High activity at 48 °C, ideal for RNA templates with complex secondary structures Produces higher specific cDNA yield compared with first-generation RT enzymes (37 °C) |
| Package | 4000/10000/200000 U |
| Download Datasheet: |  |
