| Cat# | POL-011 |
| Storage | Store at -25 ~ -15°C for 2 years. |
| Activity | 1 U/μL |
| Description | High-Fidelity DNA Polymerase, based on Pyrococcus Furiosis DNA Polymerase, is genetically engineered to contain a 5′→3′ polymerase domain and a 3′→5′ exonuclease domain. This combination significantly improves the enzyme′ s thermostability and fidelity. It has a thermostability of 98°C and fidelity 52 times that of Taq DNA polymerase and 6 times that of ordinary Pfu DNA polymerase. |
| Applications | This enzyme is primarily designed for NGS library amplification, overcoming GC-bias caused by amplifying templates with varying GC content, resulting in a substantial increase in library amplification yield. |
| Specification | 100 U; 500 U |
| Activity Definitions | Using activated salmon sperm DNA as a template/primer, at 74°C for 30 min, the amount of enzyme that ingests 10 nmol of complete nucleotides as acidic insoluble matter is defined as 1 activity unit (U). |
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