| Cat# | DIA-441 |
| Description | Taq polymerase chemically treated to block activity before heating, preventing low-temperature non-specific amplification. Suitable for high-specificity PCR, multiplex PCR, high-GC content (>60%), and complex genomic amplification. Pre-heating activity inhibited to prevent non-specific amplification, suitable for complex PCR applications. |
| Unit Definition | One unit (U) is defined as the amount of enzyme required, after heat activation, to incorporate 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 74 °C. |
| Storage | –20 °C |
| Contaminants | Free of E. coli DNA residues; free of endo-/exonuclease contamination |
| Applications | High-specificity PCR; amplification of complex templates; multiplex PCR; genome amplification assays; qPCR. |
| Product Overview | Product Components: HotStart Taq DNA Polymerase (5 U/μL): 50 μL 5× HS Taq Buffer with Mg2+: 1 mL × 2 dNTPs (10 mM each): 200 μL Product Features: Convenient: matches standard hot-start enzyme PCR conditions with no program changes needed High efficiency: minimizes nonspecific bands and smears, ensuring high specificity High sensitivity: amplifies specific gene fragments from as little as 0.05 ng human genomic DNA |
| Package | 250 U |
| Download Datasheet: |  |
