| Cat# | DIA-443 |
| Description | Antibody-modified Taq DNA Polymerase with hot-start function. Prevents low-temperature non-specific amplification. Suitable for high-specificity PCR, multiplex PCR, high-GC templates (>60%), and large-scale genomic amplification. High-specificity, multiplex, and high-GC amplification suitable. |
| Unit Definition | One unit (U) is defined as the activity that incorporates 10 nmol of total nucleotides into acid-insoluble material in 30 minutes at 74 °C, using activated salmon sperm DNA as template/primer. |
| Storage | –20 °C ± 5 °C |
| Contaminants | Free of endo-/exonuclease contamination |
| Applications | High-specificity PCR; amplification of complex templates; multiplex PCR; genome amplification assays; qPCR. |
| Product Overview | Product Components: 250 U: HotStart Taq DNA Polymerase (B) (5 U/μL): 50 μL 5× HS Taq Buffer with Mg2+: 1 mL × 2 dNTPs (10mM each): 200 μL 5 KU: HotStart Taq DNA Polymerase (B) (5 U/μL): 1 mL 5× HS Taq Buffer with Mg2+: (1 mL × 2) × 5 25 KU: HotStart Taq DNA Polymerase (B) (5 U/μL): 1 mL 5× HS Taq Buffer with Mg2+: 40 mL 50 KU: HotStart Taq DNA Polymerase (B) (5 U/μL): 10 mL 250 KU: HotStart Taq DNA Polymerase (B) (5 U/μL): 50 mL Product Features: High specificity and sensitivity with strong stability Broad application range |
| Package | 250 U/5 KU/25 KU/50 KU/250 KU |
| Download Datasheet: |  |
